Hygienic microbiological testing requires a rigorous attitude and a high sense of responsibility. Any small mistake will affect the accuracy of the test. If it is a relatively important or dangerous strain, improper management may lose the strain, may also cause laboratory contamination, and even the outflow of strains, with disastrous consequences.
The laboratory will be exposed to various test samples, and different microorganisms may be brought in daily. It is indispensable to do a good job in laboratory safety protection, and form an institutionalization, strictly control the personnel entering and exiting the site, conduct regular disinfection treatment, and make detailed records. For dangerous bacterial species, management and control must be strengthened, and special personnel must be responsible for supervision. Other people should not be contacted unless inspection is necessary. All kinds of equipment, especially disinfection equipment, should be inspected and repaired regularly. Once problems are found, they should be eliminated immediately. If the inspection is affected, the inspection should be notified to terminate or re-inspect. Regularly train the laboratory personnel to use the instrument, learn the various rules and regulations of the laboratory and the handling plan in the event of an accident. While ensuring the accuracy of the experiment, the safety of the experimenters must be ensured, and various accidental injuries that may occur are reduced to a minimum.
The work of the laboratory requires numerous instruments to assist in completion. Commonly used testing equipment includes thermometers, constant temperature incubators, biological image analysis systems, PCR analysis machines, high and low power microscopes, centrifuges, and so on. How to make these instruments play the best role? Experimenters must thoroughly understand the performance, usage, and precautions of various equipment, and do maintenance and registration before and after each use to avoid damage. Ensuring that the instrument is in good condition is conducive to the accuracy and accuracy of the inspection work.
Science and technology are constantly advancing, and experimental data requirements are becoming more and more refined, even up to the nanometer level. How to improve the experimental method to make the experimental data meet the requirements? Take infectious diseases as an example. Viruses such as mad cow disease, AIDS, and Ebola have been raging for a long time. People are no longer satisfied with passive prevention and treatment. Instead, they want to thoroughly understand the virus from the molecular biology level, so as to develop genetic and molecular defenses. , Treatment measures to defeat the virus at its root. Therefore, if conditions permit, the laboratory should introduce more advanced equipment, technology and outstanding professionals.
A lot of waste materials will be generated during the experiment, such as contaminated protective equipment, redundant and useless samples and experimental equipment, etc. These waste materials cannot be directly discarded and need to be collected and sorted. The laboratory should be equipped with trash bins for holding waste, and pay attention to leakage prevention. If necessary, a certain disinfectant should be equipped for sterilization and antivirus. Disposable utensils, including needles, needles, etc., should not be used multiple times and should be burned after high-temperature disinfection. Appliances that have been used many times must be thoroughly disinfected, and then washed and dried. It needs to be checked for integrity and disinfection before each use. Glass fragments, razor blades, and needles must be handled in a centralized manner. Direct contact with hands is prohibited, and they must not be directly buried as domestic waste.
Before the test, the experimenter should understand the test object, process and various instruments that need to be used, plan the experimental steps and be familiar with the use methods and precautions of various equipment. Before and after entering the laboratory, you need to wash your hands and disinfect, remove all kinds of jewelry you wear, and tie up your long hair. Take protection according to the type of inspection. For example, wear suitable protective clothing. If there are splashes of liquid droplets, you should wear masks, goggles, and laboratory gloves. Choose waterproof protective clothing with plastic aprons. Before wearing protective clothing, check carefully whether the protective equipment is damaged or contaminated to prevent damage during the experiment. Change the protective clothing regularly. It is strictly forbidden to wear protective clothing to contact the external environment. If contamination occurs during the inspection, it needs to be replaced immediately. If it is a disposable protective clothing, it cannot be reused or discarded directly after use. It needs to be professionally disinfected and treated collectively. If it is a commonly used protective clothing, it needs to be disinfected and then washed and stored in a sterile environment. The inspection work must minimize the interference of other factors so that the experiment can be carried out in an orderly and successful manner.
In addition to equipment, the quality of laboratory personnel also needs to be improved. Strict selection and continuous training of on-duty personnel. The personnel engaged in inspection work need to continuously exquisite technology and strive to improve their professional knowledge and operational proficiency. For the problems encountered in the inspection process, we must actively search for information and seek help, and never leave it alone. On the other hand, experimenters should be more in line with international standards, understand the latest testing methods and equipment, and constantly correct their own shortcomings. In addition to mastering the experimental operations, they must also learn a complete set of testing functions, such as culture medium for different testing objects. How to make, maintenance of various instruments, working principle and simple maintenance methods, etc.
The most important thing to do well in experiments is mentality. The laboratory must do a good job in guiding the thoughts of experimenters. Every step of the experiment is equally important and will affect the direction of the results of the experiment. Experimenters should have a sense of awe for science, be serious in the experiment process, strictly abide by the laboratory system, and do the experiment steadily.